Question 1

What is the history and background of Embryotools?

Accepted Answer

Embryotools was founded in Barcelona by Dr. Nuno Costa‑Borges, PhD, and Dr. Gloria Calderón, PhD, both internationally recognized embryologists with more than 30 years of combined experience in clinical and animal reproduction. Since its inception, the company has grown into a leading biotechnology centre that provides training, quality‑control testing, consulting and equine reproduction services. Key milestones include the first successful animal cloning in Spain (2009) and the world’s first foal born via maternal spindle transfer (2025), a breakthrough that also demonstrated the technique’s potential for human IVF. Embryotools’s laboratory is ISO‑17025 accredited and audited annually by the Spanish Accreditation Body (ENAC), reflecting its commitment to the highest standards. Today, the organization supports IVF clinics worldwide through cutting‑edge research, advanced laboratory technologies and a dedicated team of fertility specialists and scientists.

Question 2

Who are the fertility doctors and specialists at Embryotools?

Accepted Answer

Embryotools’s scientific team is led by Dr. Nuno Costa‑Borges, PhD, who serves as CEO, CSO and co‑founder and is a senior embryologist with more than 15 years of clinical and research experience. The former president, Dr. Gloria Calderón, PhD, is a renowned clinical embryologist who helped achieve Spain’s first IVF pregnancies and now acts as a senior consultant and guest lecturer. The laboratory is directed by Enric Mestres, PhD, who also manages quality assurance as the Quality Manager. Additional specialists include Mònica Acacio, MSc, head of the Veterinary Center and research programs; Queralt Matia, MSc, head of the Products Center; Carolina Castelló, BSc, who coordinates the Training Center and Clinical Consulting; and Andrea Villamar, a technical lead for the Quality Control Center. Together, this multidisciplinary group of fertility specialists, reproductive embryologists and quality experts provides consulting, training and research services to IVF laboratories worldwide.

Question 3

What IVF laboratory technology does Embryotools use?

Accepted Answer

Embryotools operates a state‑of‑the‑art IVF laboratory that complies with ISO‑17025 accreditation and GLP standards, ensuring rigorous quality and reproducibility. Core technologies include the Mouse Embryo Assay (MEA) for toxicity screening, offered in standard, cell‑count and time‑lapse (MET) formats, which provides high‑resolution monitoring of embryo development to the blastocyst stage. The facility employs advanced vitrification systems for oocyte, embryo and blastocyst cryopreservation, as well as precision ICSI micromanipulators for intracytoplasmic sperm injection. Temperature and pH are continuously recorded using high‑precision sensors, and the lab integrates time‑lapse imaging platforms to assess embryo morphology and growth dynamics. All equipment is selected for compatibility with assisted reproduction protocols, supporting embryo transfer and blastocyst culture in both human and animal IVF programs.

Question 4

What fertility treatments and services does Embryotools offer?

Accepted Answer

Embryotools delivers a comprehensive portfolio of assisted reproduction services aimed at IVF laboratories and animal breeding programs. Its IVF Training Center offers hands‑on courses covering ICSI, embryo biopsy, blastocyst grading, vitrification of oocytes, embryos and blastocysts, and pre‑implantation genetic testing (PGT) protocols, all taught by experienced embryologists. The Quality Control Center provides ISO‑17025 accredited assays such as the Mouse Embryo Assay (MEA) with standard, cell‑count and time‑lapse (MET) options to evaluate culture media, consumables and equipment. Consulting services include laboratory design, workflow optimization, temperature and pH monitoring, and implementation of quality‑control systems to improve clinical outcomes of in vitro fertilization cycles. In the equine sector, Embryotools offers ICSI, ovum‑pick‑up (OPU), and maternal spindle transfer (MST) for elite horses, delivering high‑success‑rate embryo production and cryopreservation. The R&D unit develops novel techniques, while the product line supplies specialized devices such as the e‑tubing™ PCR tube holder and cryopreserved mouse embryos or hamster oocytes for training and research.

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